FabAct™ Proteinase K (recombinant), Molecular Grade (Liquid)

FabAct™ Proteinase K (recombinant), Molecular Grade (Liquid)

Information

Name

FabAct™ Proteinase K (recombinant), Molecular Grade (Liquid)

Catalog number

9213-25

Price

458 €

Size

25 ml

Detailed information

Background

A highly reactive serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is the most powerful proteinase among all proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic or hydrophobic amino acids. The application of the molecular grade FabAct™ Proteinase K is similar to the Native Proteinase K and is used in the isolation or preparation of high molecular weight nucleic acids. However, FabAct™ Proteinase K is highly pure and has a higher specific activity and is more stable at room temperature as compared to native Proteinase K. It is stable and active over a wide pH range of 4-12. It can be used on any situation to digest native and denatured proteins. FabAct™ Proteinase K is also active with SDS, urea and EDTA and the most active temperature is 65°C. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).

Gene

Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).

Unit

One unit is defined as the amount of protease that cleaves the FITC-labeled casein substrate to yield an amount of fluorescence equivalent to 1.0 nmol of unquenched FITC per minute at 37°C, pH 8.0

Synonym

Protease K, Endopeptidase K, Tritirachium alkaline proteinase

Other name

Protease K, Endopeptidase K, Tritirachium alkaline proteinase

How to reconstitute

50 mM Tris-HCl (pH 7.5), 3 mM CaCl2, 50% Glycerol

Physical properties

50 mM Tris-HCl (pH 7.5), 3 mM CaCl2, 50% Glycerol

Notes

For Research Use Only! Not to be used in humans

Source

Tritirachium album limber gene, recombinant

Before use

Centrifuge the vial prior to opening.

Tested applications

Native PAGE and SDS PAGE, N/A

Additional source

Recombinants or rec. proteins

Group

recombinants

Tested activity

1.07 kU/mg

Concentration

20 mg/ml

Molecular weight

29.3 kDa

Shipping under

Gel Pack

Physical appearance

Liquid

Purification

≥99%

Storage condition

-20°C

NCBI number

P06873

NCBI gene number

5621

NCBI gene

PROK

Gene source

N/A

Level of endotoxin

N/A

Aa sequence

N/A

Recombinant

Yes

Binding ability

N/A

Reesults

N/A

Biological activity

N/A

Copyright © 2008-2021 Labograde