Bovine Serum Albumin – Heat Shock, Reagent Grade, pH 7.0
SOURCE: The raw material used to manufacture this lot was derived exclusively from USDA-regulated abattoirs located in the United States. The animals received ante-mortem and post-mortem inspection under a licensed veterinarian’s supervision and were certified at the time of slaughter as free of infectious or contagious diseases and injurious parasites. PURITY: ≥ 96% by SDS-PAGE analysis MOL. WEIGHT: ~ 66.43 kDa FORM: Off-white powder ALBUMIN CONTENT: ≥ 98% by electrophoresis. pH: 7.0 ± 0.2 MOISTURE: ≤ 5.0% HEAVY METALS: ≤ 10 ppm ASH: ≤ 2% IgG: Not detected. SOLUBILITY: ~ 50 mg/ml in water, with 15 min manual stirring. APPLICATIONS/ADVANTAGES: • Nutrient in tissue culture media. • Protein standard. • Negative for globulins that may interfere with assays.
This BSA was purified by a proprietary heat shock process. BSA makes up approximately 60% of all proteins in animal serum. It is commonly used in cell culture protocols, particularly when protein supplementation is necessary and the other components of serum are unwanted. It aids in stabilization of enzymes and other proteins to prevent proteolysis and denaturation, especially when the proteins are present in low concentrations. In cell culture its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger for removing toxic substances, including pyrogens, from the medium.
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Serum should be stored at +5°C