H3K27ac, Histone H3 acetylated lysine 27 (ChIP grade)
AS16 3195
615 €
50 µg
AS16 3190-10 | anti-H3K4me3 | histone H3, trimethylated lysine 4 (H3K4me3) (10 µg), rabbit antibodies AS16 3187 | anti-H3K56me1 | Histone H3 (monomethyl Lys56), polyclonal antibodies AS16 3182 | anti-H3K18me2 | Histone H3 (dimethyl Lys18), rabbit antibodies AS16 3183 | anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies AS16 3181 | anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS16 3197 | anti-anti-H3core, monoclonal antibodiesAS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics
Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The agisera antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials.
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.
chromatin immunoprecipiation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), immunofluorescence (IF), peptide array (PA), western blot (WB)
0.5-5 µg/IP (ChIP/ChIP-seq), 1: 20 000 (Dot), 1: 500 (ELISA), 1: 500 (IF), 1: 1000 (WB)
Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300.
Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
No confirmed exceptions from predicted reactivity known at the moment
To be added when available, antibody released in February 2016.
Affinity purified serum in PBS with 0.05 % sodium azide
See included datasheet or contact our support service
KLH-conjugated synthetic peptide
A.thaliana, mouse, rat
Refer to NCBI
Polyclonal
Polyclonal
Inquire
Rabbit
P68431
15 kDa
human