anti-Dnmt3b (mouse) antibody affinity-pure (rabbit polyclonal), CHIP grade, WB, IP, IF, IHC
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by AS ONE INTERNATIONAL. Rabbit antibodies are very stable and can be stored for several days at room temperature. AS ONE INTERNATIONAL adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
A high affinity purification column was use to purify anti-Dnmt3b (mouse) antibody -pure (rabbit polyclonal) CHIP grade, IP, IF, IHC by AS ONE INTERNATIONAL by chromatographic size exclusion.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
If you buy Antibodies supplied by AS ONE INTERNATIONAL they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The AS ONE INTERNATIONAL antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The ultrapure anti-Dnmt3b (mouse) antibody -pure (rabbit polyclonal) CHIP grade, IP, IF, IHC supplied from AS ONE INTERNATIONAL is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
Mus musculus,Oryctolagus cuniculus
Polyclonals and antibodies