Bovine Serum Albumin – Cohn Fraction V, Immunoassay Grade, Protease Free, pH – 5.2
SOURCE: The raw material used to manufacture this lot was derived exclusively from USDA-regulated abattoirs located in the United States. The animals received ante-mortem and post-mortem inspection under a licensed veterinarian’s supervision and were certified at the time of slaughter as free of infectious or contagious diseases and injurious parasites. PURITY: ≥ 98% by SDS-PAGE gel MOL. WEIGHT: ~ 66.43 kDa ENDOTOXIN LEVEL:> 0.3 EU/mg by Gel Clot FORM: Light powder OTHER PARAMETERS: pH (1% solution): 5.2 by pH meter analysis Calcium Content: <2 mg/dL by Aesenazo III analysis Chloride Content: <25 mEq/L by ISE analysis Potassium Content: <0.2 mEq/L by ISE analysis Sodium Content: <20 mEq/L by ISE analysis Fatty Acid: 1.1 mg/gm by Enzymatic colorimetric analysis Protease Content: BDL by EIA Moisture Content: 0.8 % by thermogravimetric analysis Microbial Content: Passed lower limit. Growth monitored on a plate at 30-35ºC for 48 hrs. Mycoplasma content: Negative by E.I.A. Qualitative Viral Agents (9 CFR 113): None Detected. SOLUBILITY: 50 mg/ml in water, with 15 min of manual stirring. APPLICATIONS/ADVANTAGES: • Can be used in most systems which do not require a protease free or enzyme free reagent. • Ideal for making diluents, controls and calibrators/standards. • Applicable as a protein base in diagnostic reagents or where no proteolytic activity is essential. • Reconstitutes easily into an optically clear and easily filtered solution.
BioVision’s BSA is prepared by utilizing the Cohn Cold Ethanol Precipitation Process. This process is optimal for preserving the nutrient factors critical to the applications of cell culture, leptospira and microbiological growth. BSA makes up approximately 60% of all proteins in animal serum. It is commonly used in cell culture protocols, particularly when protein supplementation is necessary and the other components of serum are unwanted. It aids in stabilization of enzymes and other proteins to prevent proteolysis and denaturation, especially when the proteins are present in low concentrations. In cell culture its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger for removing toxic substances, including pyrogens, from the medium.
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Serum should be stored at +5°C