Immunization Grade Human Type II Collagen, 1 mg, lyophilized
R. B. Clague, K. Morgan, T. I. Reynolds, H. J. Williams. The prevalence of serum IgG antibodies to Type II Collagen in American patients with rheumatoid arthritis. British Journal of Rheumatology, 1994;33:336338 Kim W.U., Yoo W.H., Won Park, Kang Y.M., Kim S.I., Park J.H., Lee S.S., Joo Y.S, Min J. K., Hong Y.S., Lee S.H., Park S.H., Cho C.S., Kim H.Y. IgG antibodies to type II collagen reflect inflammatory activity in patients with rheumatoid arthritis. Journal of Rheumatology, 2000; 27(3):575581. Terato K., DeArmey D.A., Ye X.J., Griffiths M.M., Cremer M.A. The Mechanism of Autoantibody Formation to Cartilage in Rheumatoid Arthritis: Possible CrossReaction of Antibodies to Dietary Collagens with Autologous Type II Collagen. Clin Imm Immunopath, 1996; 79(2):142154(13) Terato K, Shimozuru Y, Katayama K, et al. Specificity of antibodies to type II collagen in rheumatoid arthritis. Arthritis Rheum 1990;33:1493500.
The term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )