![Avidin D, Cell Sorting Grade (FITC)[Avidin D, Cell Sorting Grade]](/images/smoothy/products/slider/9.jpg)
Avidin D, Cell Sorting Grade (FITC)[Avidin D, Cell Sorting Grade]
MBS634282
5 €
NA
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
For cells, cell lines and tissues in culture till half confluency.
Anti-FITC Antibody
Protein
cell