Real-time PCR detection kit for Dobrava-Belgrade virus
50 Reactions (Easy Version-Require PCR Machine)
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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PCR, polymerase chain reaction