Polystyrene Particle Size Standard, Flow Cytometry Grade, 2.5x1E6/mL, 3.0-3.4µm, 5mL

Polystyrene Particle Size Standard, Flow Cytometry Grade, 2.5x1E6/mL, 3.0-3.4µm, 5mL

Information

Name

Polystyrene Particle Size Standard, Flow Cytometry Grade, 2.5x1E6/mL, 3.0-3.4µm, 5mL

Catalog number

PPS-2

Price

300 €

Size

2X5mL

Detailed information

Test

Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

Description

FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.

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