Carboxyl Fluorescent Particles, UltraBlue, Flow Cytometry Grade, 1E7/mL, 5.0-5.9 um, 2 mL
FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
What makes our Fluorescent Particles excellent tools for bioimaging and biosensing is their stability and uniformal fluorescence. We can supply fluorescent particles with functional groups for covalent binding of proteins, peptides and antibodies. We can supply particles covered with functuonal groups such as Amino groups, Carboxyl groups, Dimethylamino groups, etc. The SPHERO fluorescent microparticles are produced by staining polystyrene particles with a fluorophore solution or by polymerizing a fluorophore in styrene in the presence of polystyrene core particles. This way an enormous variety of fluorescent particles can be made, with a large range in terms of size, fluorophores, intensity of the fluorescence and various surface functional groups. The fluorophores of our SPHERO fluorescent particles are NOT soluble in water which gives them a great deal of stability. If strored properly, the fluorophores that are incorporated in the particles will not blanch and lose their colour and/or intensity of fluorescence.
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
These particles can be used for calibration with BD FACSes like FACSCalibur, FACSCanto, Beckman Coulter Flow Cytometer, Becton Dickinson FACScan, Becton Dickinson Flow Cytometers, Miltenyi cytometer, Millipore Flow Cytometers and Millipore Guava.
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