Polystyrene Particle Size Standard Kit, Flow Cytometry Grade, 2.5 x 1E6/mL, 6x5 mL, 2.0-2.4 µm, 3.0-3.4 µm, 5.0-5.9 µm, 7.0-7.9 µm, 8.0-12.9 µm, & 13.0-17.9 µm
& 13.0-17.9 µm
GENTAUR offers a wide variety of crosslinked polystyrene particles: non-uniform, uniform shape, and porous crosslinked polystyrene. The cheaper non-uniform particles are useful in the cases when the shape of the particles is of no importance to the application. If you need perfectly spherical monosized polymer particles, we recomend the use of the 0.5% DVB cross-linked polystyrene particles. Uniform cross-linked polystyrene particles that are stable in the presence of organic solvents are also supplied by GENTAUR. If you need additional surface area then the recomended particles are the porous crosslinked beads. Cross-linked polystyrene particles of larger sizes are also available on request.
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
These particles can be used for calibration with BD FACSes like FACSCalibur, FACSCanto, Beckman Coulter Flow Cytometer, Becton Dickinson FACScan, Becton Dickinson Flow Cytometers, Miltenyi cytometer, Millipore Flow Cytometers and Millipore Guava.
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2.0-2.4 µm, 3.0-3.4 µm, 5.0-5.9 µm, 7.0-7.9 µm, 8.0-12.9 µm, & 13.0-17.9 µm
2.5 x 1E6/mL