H3K36me3, Histone H3 trimethylated lysine 36 (ChIP grade)

H3K36me3, Histone H3 trimethylated lysine 36 (ChIP grade)

Information

Name

H3K36me3, Histone H3 trimethylated lysine 36 (ChIP grade)

Catalog number

AS16 3196

Price

628 €

Size

50 µg

Detailed information

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Properties

Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The agisera antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials.

Storage condition

Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Scientific context

Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K36 is associated with active genes.

Verified applications

chromatin immunoprecipiation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), immunofluorescence (IF), peptide array (PA), western blot (WB)

Recommended dilutions for use

0.5-5 µg/IP (ChIP/ChIP-seq), 1: 20 000 (Dot), 1: 4000 (ELISA), 1: 500 (IF), 1: 10 000 (PA), 1: 1000 (WB)

Supplementary information

Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300.

Notes

Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.

No reactivity

No confirmed exceptions from predicted reactivity known at the moment

References

To be added when available, antibody released in February 2016.

Purification

Affinity purified serum in PBS with 0.05 % sodium azide

How to reconstitute

See included datasheet or contact our support service

Immunogen

KLH-conjugated synthetic peptide

Possible reactivity

A.thaliana, mouse, O. sativa

TAIR number

Refer to NCBI

Clone

Polyclonal

Clonality

Polyclonal

Available ordering format

Inquire

Raised in

Rabbit

Protein number

P68431

Molecular weight (expected | аpparent)

15 kDa

Verified reactivity

human

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